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Transposon-derived small RNA is responsible for modified function of WRKY45 locus

信息来源:作物遗传改良国家重点实验室    发布时间:2016-06-30
  • Haitao Zhang
  • , Zeng Tao
  • , Hanming Hong
  • , Zhihui Chen
  • , Changyin Wu
  • , Xianghua Li
  • , Jinghua Xiao
  •  & Shiping Wang
    • Nature Plants 2, Article number: 16016 (2016)
    • doi:10.1038/nplants.2016.16
    • Abstract

      Transposable elements (TEs) are an important source for generating small interfering RNAs (siRNAs) in plants and animals. Although TE-siRNA-induced silencing of TEs by RNA-directed DNA methylation (RdDM) in the maintenance of genome integrity has been intensively studied, it is unknown whether this type of silencing occurs in suppressing endogenous non-TE genes during host–pathogen interactions. Here we show that a TE-siRNA, TE-siR815, causes opposite functions for the two alleles, WRKY45-1 and WRKY45-2, of the WRKY45 transcription factor in rice resistance to Xanthomonas oryzae pv. oryzae, which causes the most devastating bacterial disease in rice worldwide. Expression of WRKY45-1, but notWRKY45-2, generated TE-siR815, which in turn repressed ST1, an important component in WRKY45-mediated resistance, by RdDM. Suppression of ST1abolished WRKY45-mediated resistance leading to pathogen susceptibility. These results suggest that TE-siR815 contributes to the natural variation of the WRKY45locus and TE-siR815-induced suppression of ST1 results in the negative role ofWRKY45-1 but positive role of WRKY45-2 in regulating disease resistance.

       

      全文链接:

      http://www.nature.com/articles/nplants201616

 

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