当前位置:首页 >> 正文内容

Multi-omics maps of cotton fibre reveal epigenetic basis for staged single-cell differentiation

信息来源:作物遗传改良国家重点实验室    发布时间:2016-06-30
  1. Maojun Wang, 
  2. Pengcheng Wang, 
  3. Lili Tu, 
  4. Sitao Zhu, 
  5. Lin Zhang, 
  6. Zhonghua Li,
  7. Qinghua Zhang, 
  8. Daojun Yuan and 
  9. Xianlong Zhang*

Nucl. Acids Res. (2016)doi: 10.1093/nar/gkw238

 

 

Abstract

Epigenetic modifications are highlighted for their great importance in regulating plant development, but their function associated with single-cell differentiation remains undetermined. Here, we used the cotton fibre, which is the epidermal hair on the cotton ovule, as a model to investigate the regulatory role of DNA methylation in cell differentiation. The level of CHH (H = A, T, or C) DNA methylation level was found to increase during fibre development, accompanied by a decrease in RNA-directed DNA methylation (RdDM). Examination of nucleosome positioning revealed a gradual transition from euchromatin to heterochromatin for chromatin dynamics in developing fibres, which could shape the DNA methylation landscape. The observed increase in DNA methylation in fibres, compared with other ovule tissue, was demonstrated to be mediated predominantly by an active H3K9me2-dependent pathway rather than the RdDM pathway, which was inactive. Furthermore, integrated multi-omics analyses revealed that dynamic DNA methylation played a role in the regulation of lipid biosynthesis and spatio-temporal modulation of reactive oxygen species during fibre differentiation. Our study illustrates two divergent pathways mediating a continuous increase of DNA methylation and also sheds further light on the epigenetic basis for single-cell differentiation in plants. These data and analyses are made available to the wider research community through a comprehensive web portal.

 

中国·湖北·武汉 南湖狮子山街一号 430070 Tel:+86-27-87282027 Fax:+86-27-87384670

CopyRight (C) 2014 版权所有 华中农业大学作物遗传改良国家重点实验室 技术支持:阳光飞工作室