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Evolutionarily Distinct BAHD N-Acyltransferases Are Responsible for Natural Variation of Aromatic Amine Conjugates in Rice

信息来源:作物遗传改良国家重点实验室    发布时间:2016-08-10
  1. Meng Penga,1, 
  2. Yanqiang Gaoa,1, 
  3. Wei Chena,1, 
  4. Wensheng Wanga,
  5. Shuangqian Shena, 
  6. Jian Shia, 
  7. Cheng Wanga, 
  8. Yu Zhanga, 
  9. Li Zoua,
  10. Shouchuang Wanga, 
  11. Jian Wana, 
  12. Xianqing Liub, 
  13. Liang Gonga and
  14. Jie Luoa,2

Plant Cell, 2016

  • Received April 5, 2016.
  • Revised June 1, 2016.
  • Accepted June 23, 2016.
  • Published June 27, 2016.

Abstract

  • Phenolamides (PAs) are specialized (secondary) metabolites mainly synthesized by BAHD N-acyltransferases. Here, we report metabolic profiling coupled with association and linkage mapping of 11 PAs in rice (Oryza sativa). We identified 22 loci affecting PAs in leaves and 16 loci affecting PAs in seeds. We identified eight BAHD N-acyltransferases located on five chromosomes with diverse specificities, including four aromatic amine N-acyltransferases. We show that genetic variation in PAs is determined, at least in part, by allelic variation in the tissue specificity of expression of the BAHD genes responsible for their biosynthesis. Tryptamine hydroxycinnamoyl transferase 1/2 (Os-THT1/2) and tryptamine benzoyl transferase 1/2 (Os-TBT1/2) were found to be bifunctional tryptamine/tyramineN-acyltransferases. The specificity of Os-THT1 and Os-TBT1 for agmatine involved four tandem arginine residues, which have not been identified as specificity determinants for other plant BAHD transferases, illustrating the versatility of plant BAHD transferases in acquiring new acyl acceptor specificities. With phylogenetic analysis, we identified both divergent and convergent evolution of N-acyltransferases in plants, and we suggest that the BAHD family of tryptamine/tyramine N-acyltransferases evolved conservatively in monocots, especially in Gramineae. Our work demonstrates that omics-assisted gene-to-metabolite analysis provides a useful tool for bulk gene identification and crop genetic improvement.

 

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