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PMS1T, producing phased small-interfering RNAs, regulates photoperiod-sensitive male sterility in rice

信息来源:作物遗传改良国家重点实验室    发布时间:2016-12-14

PMS1T, producing phased small-interfering RNAs, regulates photoperiod-sensitive male sterility in rice

 

  • Yourong Fan, doi: 10.1073/pnas.1619159114

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    1. Yourong Fana, 
    2. Jiangyi Yanga,1, 
    3. Sandra M. Mathionib,2, 
    4. Jinsheng Yua,3, 
    5. Jianqiang Shena, 
    6. Xuefei Yanga,
    7. Lei Wanga, 
    8. Qinghua Zhanga, 
    9. Zhaoxia Caia, 
    10. Caiguo Xua, 
    11. Xianghua Lia, 
    12. Jinghua Xiaoa, 
    13. Blake C. Meyersb,2, and 
    14. Qifa Zhanga,4
    15. Significance

      New discoveries have been continuously made in recent years on the roles of noncoding RNAs in regulating biological processes. Phased small-interfering RNAs (phasiRNAs) may be the newest member discovered in recent years. The photoperiod-sensitive male sterility (PSMS) rice is a very valuable germplasm that started the era of two-line hybrid rice. Here we show that phasiRNAs generated by a long-noncoding RNA PMS1T encoded by the Pms1 locus regulates PSMS in rice. This work provides a case associating the phasiRNAs with a biological trait, especially an agriculturally highly important trait, thus confirming that the phasiRNAs indeed have biological functions.

       

      Abstract

      Phased small-interfering RNAs (phasiRNAs) are a special class of small RNAs, which are generated in 21- or 24-nt intervals from transcripts of precursor RNAs. Although phasiRNAs have been found in a range of organisms, their biological functions in plants have yet to be uncovered. Here we show that phasiRNAs generated by the photopheriod-sensetive genic male sterility 1 (Pms1) locus were associated with photoperiod-sensitive male sterility (PSMS) in rice, a germplasm that started the two-line hybrid rice breeding. The Pms1 locus encodes a long-noncoding RNA PMS1T that was preferentially expressed in young panicles. PMS1T was targeted by miR2118 to produce 21-nt phasiRNAs that preferentially accumulated in the PSMS line under long-day conditions. A single nucleotide polymorphism in PMS1Tnearby the miR2118 recognition site was critical for fertility change, likely leading to differential accumulation of the phasiRNAs. This result suggested possible roles of phasiRNAs in reproductive development of rice, demonstrating the potential importance of this RNA class as regulators in biological processes.

     

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