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Identification and analysis of adenine N6-methylation sites in the rice genome

信息来源:作物遗传改良国家重点实验室    发布时间:2018-08-03
 

 

Chao Zhou, Changshi Wang, Hongbo Liu, Qiangwei Zhou, Qian Liu, Yan Guo, Ting Peng, Jiaming Song, Jianwei Zhang, Lingling Chen, Yu Zhao, Zhixiong Zeng* and Dao-Xiu Zhou

NATURE PLANTS | ttps://doi.org/10.1038/s41477-018-0214-x

 

Abstract:

DNA N6-methyladenine (6mA) is a non-canonical DNA modi-fication that is present at low levels in different eukaryotes18, but its prevalence and genomic function in higher plants are unclear. Using mass spectrometry, immunoprecipitation and validation with analysis of single-molecule real-time sequenc-ing, we observed that about 0.2% of all adenines are 6mA methylated in the rice genome. 6mA occurs most frequently at GAGG motifs and is mapped to about 20% of genes and

14% of transposable elements. In promoters, 6mA marks silent genes, but in bodies correlates with gene activity. 6mA overlaps with 5-methylcytosine (5mC) at CG sites in gene bodies and is complementary to 5mC at CHH sites in transpos-able elements. We show that OsALKBH1 may be potentially involved in 6mA demethylation in rice. The results suggest that 6mA is complementary to 5mC as an epigenomic mark in rice and reinforce a distinct role for 6mA as a gene expression-associated epigenomic mark in eukaryotes.

 

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