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The cytoplasmic synthesis and coupled membrane translocation of eukaryotic polyphosphate by signal-activated VTC complex
2023-02-10

Zeyuan GuanJuan ChenRuiwen LiuYanke ChenQiong XingZhangmeng DuMeng ChengJianjian HuWenhui ZhangWencong MeiBeijing WanQiang WangJie ZhangPeng ChengHuanyu CaiJianbo CaoDelin ZhangJunjie YanPing YinMichael Hothorn, Zhu Liu

Nature Communicationsvolume14, Article number: 718 (2023), Published: 09 February 2023

Abstract

Inorganic polyphosphate (polyP) is an ancient energy metabolite and phosphate store that occurs ubiquitously in all organisms. The vacuolar transporter chaperone (VTC) complex integrates cytosolic polyP synthesis from ATP and polyP membrane translocation into the vacuolar lumen. In yeast and in other eukaryotes, polyP synthesis is regulated by inositol pyrophosphate (PP-InsP) nutrient messengers, directly sensed by the VTC complex. Here, we report the cryo-electron microscopy structure of signal-activated VTC complex at 3.0 Å resolution. Baker’s yeast VTC subunits Vtc1, Vtc3, and Vtc4 assemble into a 3:1:1 complex. Fifteen trans-membrane helices form a novel membrane channel enabling the transport of newly synthesized polyP into the vacuolar lumen. PP-InsP binding orients the catalytic polymerase domain at the entrance of the trans-membrane channel, both activating the enzyme and coupling polyP synthesis and membrane translocation. Together with biochemical and cellular studies, our work provides mechanistic insights into the biogenesis of an ancient energy metabolite.

文章链接:https://www.nature.com/articles/s41467-023-36466-4