Runxin Gao#, Minrong Guo#, Shiping Luan#, Weizhi Ouyang*, Xingwang Li*

Genome Biology, Volume 26, article number 390, (2025), Published: 17 November 2025

Abstract

Multivalent interactions between proteins with intrinsically disordered regions or prion-like domains can drive liquid–liquid phase separation (LLPS) and form membraneless condensates essential for diverse cellular functions. Here, we predict phase separation scores for all annotated rice proteins and present ricePSP (https://ricepsp.github.io/), a database of phase separation-associated proteins. AlphaFold structural predictions further validate the phase separation potential of these proteins. As a proof of concept, we apply ricePSP to identify flowering-related phase separation proteins, revealing insights into how LLPS may regulate flowering. Collectively, ricePSP provides a valuable resource for studying crop phase separation proteins and LLPS-related mechanisms in crop trait regulation.

原文链接：https://doi.org/10.1186/s13059-025-03842-w